Objective Type VI collagen is a major component of the pericellular matrix compartment in articular cartilage and shows severe alterations in osteoarthritic cartilage degeneration.
The positively charged DASPMI ion readily penetrates the mitochondria and is distributed between the external space and the matrix compartment in accordance to the Nernst equation.
In contrast the more loosely organised compartment known as the FRM, accounting for approximately 95% of the total volume of matrix, is thought to represent the interterritorial matrix compartment in vivo [ 25, 34].
We have previously shown that LRRK2 localizes in part to the mitochondrial outer membrane and matrix compartment in mammalian cells and rodent brain by confocal microscopy, submitochondrial fractionation and electron microscopy (12, 39).
The assay is performed using a "sandwich" format in which dermal fibroblasts are plated at the interface between an upper and lower matrix compartment in the presence of an isotropic distribution of test mitogen.
Similarly, silencing of QIL1 specifically in neurons using the Elav-Dcr-Gal4 driver to express UAS-ControlRNAi or UAS-QIL1RNAi led to loss of CJs and the formation of concentric stacks of IM inside the matrix compartment in neurons at neuromuscular junctions.
