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Exact(9)
It is worth noticing that a number of the matrix cells were empty, since no MEP was obtained from the corresponding scalp positions and muscles.
To detect F-actin in cells in Matrigel matrix, cells were fixed for 30 minutes with 4% paraformaldehyde-0.25 paraformaldehyde-0.25% on ice and then treated for 30 minutes with 0.2% Triton-X 100 in PBS.
Examination of Cre protein expression on back skin sections revealed that Cre is expressed in the bulge epithelium but not in the hair bulb, suggesting that lacZ-positive hair bulb matrix cells were likely derived from bulge cells that experienced Cre-induced recombination.
In the case of cells proliferated in the 3D matrix, cells were first magnetically separated as described above.
One to two hours after plating on matrix cells were either lysed for biochemical analysis or immunostained.
In the case of the fibrin matrix, cells were observed throughout the scaffold with a tendency to accumulate at the inner core of the material.
Similar(51)
Initially, the matrix cells are initialized as follows.
Proportion of correctly reproduced matrix cells was analyzed.
Matrix cells are found in all thalamic nuclei, but predominate in intralaminar and other nonspecific nuclei; core cells are concentrated in the specific sensory relay nuclei.
It is noteworthy that the proliferation markers Ki67 or BrdU were detected in the ORS of hair follicles, but strikingly absent in the area of the follicles where matrix cells are expected to be located at 9 days after birth.
The absence of apoptosis in the Pofut1/Tgfb3-Cre hair bulb at P30 indicated that the reduction in the number of BrdU-positive cells detected in the mutant matrix cells is not due to increased apoptosis.
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