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Initially, the matrix cells are initialized as follows.
Matrix cells are found in all thalamic nuclei, but predominate in intralaminar and other nonspecific nuclei; core cells are concentrated in the specific sensory relay nuclei.
It is noteworthy that the proliferation markers Ki67 or BrdU were detected in the ORS of hair follicles, but strikingly absent in the area of the follicles where matrix cells are expected to be located at 9 days after birth.
Initially, the first row and column of the matrix cells are set to 0, i.e. c 0, j = 0, c i, 0 = 0.
At the anagen/catagen transition, HF matrix cells are subjected to apoptosis and the DP retracts upward towards the bulge along with the dying epithelial strand.
Because transient amplifying cells have a limited potential to proliferate and because the majority of matrix cells are actively involved in continuous replication [ 4], they eventually exhaust their proliferative capacity and undergo terminal differentiation.
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Proportion of correctly reproduced matrix cells was analyzed.
It is worth noticing that a number of the matrix cells were empty, since no MEP was obtained from the corresponding scalp positions and muscles.
The absence of apoptosis in the Pofut1/Tgfb3-Cre hair bulb at P30 indicated that the reduction in the number of BrdU-positive cells detected in the mutant matrix cells is not due to increased apoptosis.
To detect F-actin in cells in Matrigel matrix, cells were fixed for 30 minutes with 4% paraformaldehyde-0.25 paraformaldehyde-0.25% on ice and then treated for 30 minutes with 0.2% Triton-X 100 in PBS.
The total number of matrix cells was 648.
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