GO localisation analysis revealed that the identified proteins from KSR1-Doxo and KSR1-Etop datasets are disseminated in numerous cellular components, including extracellular matrix, cell membrane, cytoplasm, nuclei, synapse and other organelles (Supplementary Fig. 2).
Therefore, if the pH of the extracellular matrix is lower than that of the cell cytoplasm, an intracellular-extracellular pH gradient is maintained that continuously drives HF into the cell.
Once candidate effectors are deployed, they act either in the exhaustorial matrix, the extracellular space or within the host cell cytoplasm to promote invasion and pathogenicity [ 13- 15].
C4d positive staining was seen in endothelial cell cytoplasm, as well as in astrocytoma cell cytoplasm and tissue extracellular matrix.
EVL expression was mainly found in the cell cytoplasm.
The LENA content is then released into the cell cytoplasm.
Further, BiFC revealed that Me10 interaction with TFT7 occurs in the plant cell cytoplasm.
In one hand, CCN3 proteins were detected in the extracellular matrix, conditioned cell culture medium, cytoplasm and nucleus [ 7, 10, 12], in the other hand, S100A4 has been essentially described as a cytoplasmic protein, although it was also found to be secreted [ 39].
Since there is no change in quantum yield between matrix and cytoplasm, the total fluorescence from a single cell will not change as a result of the probe translocation.
One gene (Syndecan ) encodes a transmembrane coreceptor present on all adherent cells that transduces signals from the extracellular matrix (ECM) to cytoplasm, regulating diverse cell activities (Spring et al. 1994).
