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From the BAM files and the hg38 GTF file, Qlucore Omics Explorer creates a count matrix by counting the number of reads overlapping each genomic feature in each sample (each BAM file).
The programme then made an aggregated (group) matrix by counting the individual matrices.
First, we build a co-clustering matrix by counting for each pair of patients the number of times they appear in the same group across the different solutions.
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To investigate this, we classified 20 amino acids into six types on the basis of physicochemical properties of amino acids (see Material and Methods) and made 6 * 6 amino acid property substitution matrices by counting the number of substitutions of amino acid by their types.
Each gene's upstream region is scored with matrix m by counting the number of randomly drawn genes that achieve scores equal to or greater than c m.
We also provide the position weight matrices inferred by counting nucleotide frequencies at each position within its genomic instances.
The X matrix is formed by counting the occurrences of each of the 20 amino acids in each peptide which results in a matrix with 20 columns and 255 rows.
The co-occurrence matrix is formed by counting the number of the adjacent ULBP and RIU-LBP pairs with the combination indexes, which produces the matrix with a size of 59×10.
The distance matrix was calculated by counting the number of differences.
The expression matrix was generated by counting the number of unique UMIs per gene per cell.
The characteristic ability of cancer cells to grow independently of anchorage to the surrounding solid matrix was measured by counting colony formation on soft agar for 8 to 14 days.
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