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In this work a protocol to validate analytical procedures for the quantification of drug substances formulated in polymeric systems that comprise both drug entrapped into the polymeric matrix (assay:content test) and drug released from the systems (assay:dissolution test) is developed.
Only 46% of participating laboratories used an influenza A matrix assay as well as a specific H5 assay (Table 2).
26 The presence of antibodies to hepatitis C was tested using a second generation enzyme immunoassay (Abbott Laboratories, Chicago, IL) and confirmed by the MATRIX assay (Abbott Laboratories).
A functional cell separation method called CAM, or the collagen adhesion matrix assay, was reported to detect CTCs with the invasive phenotype and to explore their molecular features [ 36].
We placed nasopharyngeal and throat swabs in viral transport media and tested them by a real time polymerase chain reaction matrix assay for influenza A using primers from the Centers for Disease Control and Prevention, Atlanta, USA.
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To evaluate the accuracy of the method on a tissue sample matrix, assays were carried out on a spiked potato sample.
An extracellular matrix invasion assay and microporous migration assay was used to assess chemotactic response and metastatic ability.
For matrix invasion assays, the haptotaxis assay above was modified such that instead of fibronectin, the basement membrane matrix mixture Matrigel (BD Biosciences, Bedford, MA) was used to cover the upper side of cell culture inserts with 8 µm pores.
The conditioned media and the cartilage explant disks were assayed for the release of biomolecules through cartilage matrix degeneration assay.
The effect of matrix on assay measurements was tested using the % recovery of the middle NIBSC spikes and the assay calibrator spikes.
To identify binding partners of nucleolin involved in monocytes differentiation, a nucleolin affinity matrix precipitation assay was performed.
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