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Matrix application protocols include manual methods such as spraying using an airbrush or TLC sprayer or dipping the tissue sections into matrix containing solutions (Schwartz et al. 2003).
Some of these artifacts related to sample preparation can be cured by improved matrix application protocols and by extended washing steps that may diminish salt and pH gradients, and these steps deserve attention to achieve well interpretable images.
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Different resin cements with different application protocols were analyzed.
File Transfer Protocol (FTP is one of the first application protocols designed for an interoperable internetwork.
A modified customized method was used for matrix application using the ImagePrep instrument.
The matrix application technique is critical to the success of a matrix-assisted laser desorption/ionization (MALDI) experiment.
Multi-modal approaches and details on matrix application methods are included in Chughtai and Heeren (2010).
For DHB, five automatic sprayer matrix application methods were developed for the automatic TM-Sprayer system.
Strict optimization of matrix application technique seems to be more critical when working with DHB.
After the matrix application, the slide was incubated in desiccator for around 5 min.
An increasing number of commercial matrix application devices have been developed recently.
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