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Irrespective of the matrix application method, a certain level of experience and practice is needed in order to obtain suitable results.
The optimized sublimation and automatic sprayer methods were directly compared to the standard, well-used airbrush matrix application method by performing MSI on serial sections of M. truncatula root nodule tissue, for both DHB and CHCA.
Previous publications reported on comparison and optimization of one matrix application method for a variety of matrices, comparison between dry coating and spray matrix application methods, or comparison between two different spray methods.
The combined use of solvent-free (sublimation) and solvent-based (automatic sprayer) matrix application techniques can provide complementary matrix peak profile results, providing the possibility to detect metabolites that were masked by interfering matrix ion peaks in one matrix application method but not masked in the other.
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Multi-modal approaches and details on matrix application methods are included in Chughtai and Heeren (2010).
For DHB, five automatic sprayer matrix application methods were developed for the automatic TM-Sprayer system.
For CHCA, six automatic sprayer matrix application methods were developed for the automatic TM-Sprayer system.
The device was used to characterize MALDI matrix application methods and evaluate their effects on chemical lateral displacement.
In this work, three matrix application methods were systematically optimized and compared: airbrush, automatic sprayer, and sublimation.
Other major changes included a modification of the matrix application methods by construction of a spraying device that allowed for improved control of the matrix deposition on tissue.
The optimized automatic sprayer method and sublimation methods reduce analyte diffusion that is typically seen with traditional airbrush matrix application methods.
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