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Fig. 3 The MALDI-MSI workflow employed here for the analysis of excised tissue (a) post-MRI consisted of freeze thaw mounting of cryosectioned tissue (b), on tissue digestion for peptide analysis (omitted for lipids) prior to matrix application, and finally generation of MALDI-MSI (c) to enable the mapping and co-registration of peaks of interest found in the corresponding MALDI spectrum (d).
This implies that the spatial structure of the cells was not affected significantly by matrix application and the measurement procedure.
Coating thickness was estimated by weighing the sample slide before and after matrix application and measuring the coated area.
Experimental parameters that should be considered include treatment of tissue immediately after sample procurement, sectioning, sample transfer to the MALDI target plate or conductive glass slide, matrix application, and tissue storage after sectioning (Caldwell and Caprioli 2005).
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Several treatment modalities have been described for ABC such as sole curettage, curettage with cementation or bone grafting, fibrosing agents (Ethiblock or phenol) or bone marrow injections, arterial embolization, adjuvant cryotherapy or radiotherapy, demineralized bone matrix applications and segmental or en bloc resections [1].
Some of these artifacts related to sample preparation can be cured by improved matrix application protocols and by extended washing steps that may diminish salt and pH gradients, and these steps deserve attention to achieve well interpretable images.
The device was used to characterize MALDI matrix application methods and evaluate their effects on chemical lateral displacement.
Figure 2 compares MS profiling spectra for pure matrix using the airbrush (blue), optimized automatic sprayer (red), and optimized sublimation (green) matrix application methods for (a) DHB and (b) CHCA.
Multi-modal approaches and details on matrix application methods are included in Chughtai and Heeren (2010).
This could be caused both by the matrix application (possibly generating delocalization) and by the thickness of the antenna.
In this work, three matrix application methods were systematically optimized and compared: airbrush, automatic sprayer, and sublimation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com