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Image analysis and base calling were performed with the standard Illumina pipeline, and with automated matrix and phasing calculations on the PhiX control that was run in the eighth lane of each flowcell.
Matrix and phasing values generated from a library control lane were applied to the lanes containing the amplified 16S fragments.
Prior to the update of Real-Time AnalySan Diegoare (IllUSAna, San Diego, USA) to version 2.2.0.2, hardcoded matrix and phasing values were used for sequencing runs.
The cDNA libraries were assigned to six individual sequencing lanes, and one dedicated PhiX control lane for matrix and phasing calculations was applied during sequencing.
The RunInfo.xml file was edited to allow for the 6 bp index to be called as a separate index read in the analysis, with matrix and phasing estimated from the PhiX control lane.
Image analysis and base calling were performed using the standard Illumina pipeline; a control library sequenced in a single lane of each flow cell was used for matrix and phasing calculations.
Similar(53)
Most polymers occur to be insoluble, in some degree, in the bitumen matrix, and phase separation may result.
First the local scattering matrix and phase matrix for the reflected and refracted waves are derived at each interface of two layers in terms of local co-ordinates.
A possible mechanism for forming ZnO NPs inside porous silica matrix and phase transformation of the ZnO/SiO2 nanocomposites into 3D architectures of Zn2SiO4 are carefully discussed.
One begins with a space or time series (η x, 0), η 0, t)) in one or two spatial dimensions (η x, y, 0), η 0, y, t)) and then computes the Riemann spectrum (the Riemann matrix and phases).
The latest Illumina HiSeq-2500 sequencer was used for sequencing, with load of ~30% PhiX for proper matrixing and phasing into the lane.
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