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These additives are not chemically bound to the polymer matrix and migrate or leach out under the influence of physicochemical factors such as temperature, sterilization and type of solvents and pH of the packaged product [12].
Cells were allowed to invade the matrix and migrate through to the other side of the membrane over 30 h at 37°C.
It is still unknown how cancer cells functionally undergo cytoskeletal rearrangement to exhibit mesenchymal features, interact with extracellular matrix, and migrate to distant locations when the EMT process is initiated.
During invasion, cells degrade or remodel the surrounding extracellular matrix and migrate through the tissue boundary (Kermorgant et al, 2001).
The ECs can now break down the matrix and migrate away from the mother vessel, producing "tracks" of intermediate ECM concentration on top of which cells have highest motility.
EMT initiates changes in gene transcription which allows epithelial cells to lose polarity and cell-cell contact, detach from the epithelial cell layer, modify the extracellular matrix and migrate through tissues.
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These revealed that, in the absence of keratins, keratinocytes adhere much faster to extracellular matrix substrates and migrate approximately two times faster compared with wild-type cells.
Tumor growth and metastasis is regulated in part by the ability of tumor cells to degrade surrounding matrix tissue and migrate.
During EMT epithelial cells break down cell-cell and cell-extracellular matrix contacts and migrate to other locations in the body [27].
Highly invasive cells are able to degrade the extracellular matrix components and migrate through the layer of Matrigel to the other side of filter by activating various proteinases.
Recent studies suggest cancer cells can circumvent the requirement for matrix proteolysis and migrate through tissues by adopting an amoeboid form of movement [ 59- 62].
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