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After hDFCs being seeded overnight on AEM, hDFCs had deposited certain amount of extracellular matrix and adhered tightly to AEM.
A biofilm has been defined as a community of microorganisms organized at interfaces, enclosed in a self-produced polymeric matrix and adhered to an inert or living tissue.
After incubation, cells that had migrated through the matrix and adhered to the other side of the inserts were fixed in 4% formalin, stained with 0.5% (weight/volume) crystal violet, and counted under a microscope.
After incubation for three days, cells which had invaded through the matrix and adhered to the other side of the insert were fixed in 4% formalin, and stained with 0.5% (w/v) crystal violet.
After culture at 37 °C in a humidified atmosphere of 5%% CO2 for 48 h, the cells that invaded the Matrigel matrix and adhered to the bottom surface of the membrane were fixed with methanol and stained with 0.5 % crystal violet.
Similar(55)
Invasiveness was evaluated by staining cells that had migrated through the extracellular matrix layer and adhered to the polycarbonate membrane at the bottom of the insert.
Cell patches with intact cell cell and cell extra cellular matrix contact attached and adhered on a scaffold within 1 h.
Biofilms are complex communities of bacteria that are encased in an extracellular matrix and adhere to almost any surface.
A true biofilm requires that yeast both form an extracellular protein matrix and adhere to a surface (Stovicek et al. 2010; Vachova et al. 2011; Bruckner and Mosch 2012).
Furthermore, the tribolayer formed during wear process, which small debris particles of chromium nitride embedded into the lubricative matrix and well adhered in the wear track, played a significant role in the anti-wear property.
The deposited nickel forms a nickel matrix and strongly adheres the Raney nickel alloy and the hydrogen absorbing alloy.
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