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These methods included Consensus method [ 30], NN-align (netMHCII-2.2) [ 31], stabilization matrix alignment method (SMM-align) [ 32], Sturniolo [ 33], average relative binding (ARB) [ 34], NetMHCIIpan [ 35], and Combinatorial library (ComLib) [ 30].
The closest structural neighbor according to distance matrix alignment (DALI) is Neisseria polysaccharea amylosucrase (PDB entry 1ZS2, 27% sequence identity), aligning with an rmsd of 2.7 Å over 492 aligned Cα positions.
We found that matrix metalloproteinase activity is not required for matrix alignment before cell migration.
Consistently, the pattern of matrix alignment depends on tissue geometry and the resulting distribution of mechanical strain, with asymmetric tissues generating a higher degree of matrix alignment along their longest axes.
Migrating cohorts use spatially localized, long-range forces and consequent matrix alignment to navigate through the ECM.
Furthermore, relative roles of topographical factors (matrix alignment vs. matrix modulus) in inducing tenogenic differentiation is not well understood.
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The sequences of human αB crystallin and other small-heat shock proteins were compared with the sequences of various tubulin proteins for homology using the dot-matrix alignment program 'Dotter' [42].
Among the nonsynonymous SNPs we identified, those that cause a dramatic protein sequence change from the standpoint of an alignment-scoring matrix (large alignment score change) may be of particular interest.
All matrices, alignments and phylogenetic trees are available in the TreeBASE database http://purl.org/phylo/treebase/phylows/study/TB2 S11451, as supplementary material, or on the webpage http://users.prf.jcu.cz/husnif00.jcu.cz/husnif00
We used a sensitive BLAST analysis along with analysis of dot-matrix alignments to look for regions of local alignment in the intergenic regions flanking developmentally important genes with similar expression in Drosophila and the other four species.
From the original matrix, 654 alignment positions could not be aligned unambiguously and so were excluded from subsequent analyses, yielding a final matrix of 4594 bp (Table 4).
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