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Some matrices were maintained with media in the absence of cells and served as negative controls.
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That means, equivalence of the new and the original matrices is maintained for a zero value of mutual impedances (hat{F}^{prime }) and (hat{H}^{prime }).
The DP matrices are D-discrete, and assume that lookup tables for efficient min-plus multiplications of these matrices are maintained as explained in the previous section.
We have also tested the effect of in situ chemical fixation of the matrices (with neutral buffered formalin) on their rigidity and found it increased the stiffness of both tumor-associated and control matrices by 22%and19%9%, respectively (E = 0.14 ± 0.06 vs. 0.09 ± 0.05 kPa, p < 0.002) (Fig. S2), while the overall stiffening of tumor-associated matrices was maintained.
Additionally, when the infiltration velocity was maintained at 1/10 of the saturated hydraulic conductivity and the matric potential at the matrix was maintained at −3 kPa, (empty) macropore bypass flow disappeared and solutes were distributed throughout the whole soil body (Mori and Higashi [2009]).
The new matrix is maintained symmetric positive definite, so that the linear system can be solved efficiently.
Similar to the original opportunistic routing protocol [36], we assume that a distance matrix is maintained by each station, which is used to calculate the distance to other stations.
The results indicate that proteins, particularly gelatin protein, can notably improve not only the mechanical properties (modulus and elasticity), but also the adhesion properties, while the high ionic conductivity of the electrolyte matrix is maintained.
Thus, nutrient supply and waste removal for cells encapsulated 3D matrix are maintained via diffusion from and to a continuous flow of fresh medium in the microchannels.
It is possible that the abnormalities of the extracellular matrix are maintaining inflammatory and autoimmune responses in late stage SSc.
5×105 NHK cells were seeded onto the collagen matrix and tissues were maintained submerged in low-calcium epidermal growth media for 2 days, submerged for 2 days in normal calcium media, and then raised to the air liquid interface for an additional 8 days by feeding from below as previously described [30].
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