Uncropped blots are available in Supplementary Fig. 12. Cell-derived matrices were generated as previously described54.
To visualise differences in microbial community structures based on species abundances, Bray-Curtis dissimilarity matrices were generated from OTU tables and subsequently subjected to PCoA.
Connectivity matrices were generated to provide an overview of all intracortical connections and subnetwork clusterings.
Common item nonequivalent groups equating design was used, hi this study 600 dichotomously responded data matrices were generated.
Alpha diversity metrics (total observed number of OTUs, predicted species (chao1), Shannon-Wiener diversity index, Simpson's Evenness and species richness estimate40) and beta diversity UniFrac distance matrices were generated from OTU tables using the QIIME pipeline.
The similarity matrices were generated with the ECFP_4 fingerprint algorithm and the Tanimoto similarity measure.
For comparison, 500 random Bernoulli measurement matrices were generated in 500 trials.
All designed matrices were generated and analyses were performed using Statistica software (version 8.0; StatSoft, Inc., http://www.statsoft.com).statsoft.com
Also, to be consistent with the previous research, matrices were generated from the Matlab code provided by the authors of [3] at http://www.di.ens.fr/~aspremon/NSPcode.html.html
Three dissimilarity matrices were generated, pocket vs. pocket using all binding site atoms (of every amino acid within a 5 Å distance from the ligand), pocket vs. pocket using binding site atoms only within 6.5 Å of the ligand, and inversely, ligand vs. ligand using the ligand atoms only.
Letter matrices were generated analogous to the digit matrices.
