The presence of other excipients within fibrous matrices was confirmed using FTIR and Raman spectroscopy.
Absence of pathogenic Y. enterocolitica in the matrices was confirmed by including a negative control sample in each test.
The presence of collagen I and various ECM proteins in the de-cellularised matrices was confirmed by immunostaining and western blotting following the protocol described in our previous studies [ 12, 13].
The presence of the studied pharmaceuticals in the polymeric matrices was confirmed by analysing the resonance signals of drug in NMR spectra, according to the literature data [ 29, 30] and spectrum obtained for pure drug.
Homogeneous distribution of f-MWCNTs and MNTs in PVDF matrix were confirmed by field emission scanning electron microscopy.
Further, changes in the expression level of genes connected with cardiac extracellular matrix were confirmed.
The stability of the enzyme in the lipid matrix was confirmed by biocatalytic activity measurements.
The chemical linkage of carbon nanotubes with polyurethane matrix was confirmed by Fourier transform infrared spectra.
Exfoliation of clay in a PA matrix was confirmed by X-ray diffraction.
