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Our objective was to compare matrices reconstituted from collagen I and Matrigel as representative barriers for ovarian cancer cell invasion.
Collectively, our experiments show that matrices reconstituted from Matrigel do not adequately reflect the barrier function of basement membranes, in either transwell or 3D invasion systems, whereas the barrier function of stromal matrices can be mimicked using polymerised collagen I.
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In contrast, MMP activity was required for invasion into 3D matrices of collagen I reconstituted from acid-soluble rat-tail collagen I, but not from pepsin-extracted collagen I (Vitrogen/Purecol), which lacks telopeptide regions.
The types of cartilage ranged from artificial, cell-free matrix substitutes based on collagen/peptide matrices [ 9] or extracted cartilage components (reconstituted from milled cartilage) [ 10] to in vitro generated, cell-containing matrices (derived from the three-dimensional (3D) culture of chondrocytes) [ 11].
Tissue models reconstituted from cells and extracellular matrix (ECM) simulate natural tissues.
Why not even stone reconstituted from the dust of the old stone?
Other information can be reconstituted from e-mail messages and other sources, Mr. Williams said.
The morels had been reconstituted from dried, because fresh ones, alas, were out of season.
Temporal MPI trends can also be reconstituted from past regulatory surveys.
tomato DC3000 a COR biosynthetic gene cluster was designed and reconstituted from synthetic DNA fragments.
Near zero-order release could be achieved for CyA-loaded nanoemulsions reconstituted from the SNEDDS.
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