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To define the composition of extracellular matrices of tissues and tumors, we have developed a proteomics-based method to enrich and identify ECM proteins and coupled it with a bioinformatic annotation of the 'matrisome' defined as the ensemble of ECM and ECM-associated proteins (Naba et al., 2012).
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However, IGFBP5 also binds to the extracellular matrix of tissues, and can thereby serve as a local reservoir of IGF-1 [ 23].
Matrix proteoglycans define matrix structure, mineralization, and resulting biomechanics of tissues and their attachment sites.
Elastin is a key extracellular matrix protein in a range of tissues and a viable candidate for elastic tissue engineering.
Also of interest are the viscoelastic (i.e. time-dependent) properties of tissues and matrix at the cellular level.
Hence, there is a need to develop methods to measure elastic properties and stiffness of tissues and matrix in a non-invasive manner for clinical application.
The similarity of the nanofibers to the natural extracellular matrix of many tissues and the importance of nanofibers in TE applications are highlighted.
Collagen type-I is a major component of the extracellular matrix of most tissues and it is increasingly utilized for surface engineering of biomaterials to accelerate receptor-mediated cell adhesion.
Electrospinning represents an attractive approach to the fabrication of fibrous biomaterials, which can mimic the size scales of fibers composing the extracellular matrix of native tissues and organs.
Engineered scaffolds produced by electrospinning of biodegradable polymers offer a 3D, nanofibrous environment with controllable structural, chemical, and mechanical properties that mimic the extracellular matrix of native tissues and have shown promise for a number of tissue engineering applications.
Electrospun materials have attracted research interests because submicron fibers are naturally found in the extracellular matrix of human tissues and organs.
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