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Hydrogels derived from natural polysaccharides are ideal scaffolds as they resemble the extracellular matrices of tissues comprised of various glycosaminoglycans (GAGs).
To define the composition of extracellular matrices of tissues and tumors, we have developed a proteomics-based method to enrich and identify ECM proteins and coupled it with a bioinformatic annotation of the 'matrisome' defined as the ensemble of ECM and ECM-associated proteins (Naba et al., 2012).
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The overarching goal for most of these biomaterials is its ability to closely mimic native extracellular matrix of tissues.
In this chapter, we intend to describe the development of electrofluidodynamic technique as a set of processes that allow obtaining a variety of hierarchical architectures based on electrospun fibers that mimic the native extracellular matrix of tissues.
However, IGFBP5 also binds to the extracellular matrix of tissues, and can thereby serve as a local reservoir of IGF-1 [ 23].
Perlecan may also occur outside the basement membranes, that is, in the extracellular matrix of tissues deprived of these structures, as in the case of the matrix of cartilage, sinusoid vessels of liver, spleen, or in the lymph nodes [ 35].
This is due to the limited fragmentation efficiency of singly charged ions, which are produced in MALDI almost exclusively, and to ion suppression effects in the complex matrix of tissue sections.
Immunohistochemically, Smoc-1 was localized in the basement membrane zones and extracellular matrices of various tissues.
These cytokines are abundant in inflamed synovium and produce marked disturbances of bone and cartilage remodeling leading to destruction of the extracellular matrices of these tissues [ 37, 38].
Another system that allows to rapidly acquire fluorescence wavelength-time matrices of biological tissues is introduced by Lloyd et al. [ 10].
The extracellular matrix (ECM) of tissues is an assembly of insoluble macromolecules that specifically interact with soluble bioactive molecules and regulate their distribution and availability to cells.
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