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Decellularizing matrices has previously been shown to (i) relax matrix networks by ~20% [ 53], and (ii) alter the ratio of DOC soluble (nascent) to DOC insoluble (crosslinked) Fn characterized herein [ 54], as DOC soluble Fn will have most likely been washed away.
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PEGT/PBT block-copolymer matrices have previously been shown as a favorable dermal substitute.
Loss of β1 integrin binding to the extracellular matrix has previously been shown to perturb FAK signaling and the MAPK/ERK kinase pathway downstream of FAK is important for cell survival and proliferation [38], [39].
For the culture conditions, Medium B used in conjunction with fibronectin as a matrix, has previously been reported to provide the best proliferative conditions for mammalian cell types in vitro[ 1][ 18].
Statins were then applied either during the fibroblast-promoted matrix remodelling phase or the SCC invasion phase when carcinoma cells move into a matrix that has previously been 'conditioned' by CAFs. Figure 2B and C shows that 1 μ M simvastatin or lovastatin treatment during the matrix remodelling phase reduces SCC invasion but treatment after this phase has no effect.
Tissue-engineered pericardium (TEP) is a collagen-rich matrix that has previously been shown to promote in vivo and in vitro tissue regeneration.
A perturbation of matrix assembly has previously been reported in association with mutant PAPSS2.
Lysyl oxidase (LOX) plays an essential role in the formation and maintenance of the extracellular matrix, and has previously been linked to increased in vitro invasion of breast cancer cells [ 1].
The distribution of analytes in MALDI matrix crystal has previously been measured with TOF-SIMS, MALDI, and confocal fluorescence microscopy; however, without a fixed origin, the chemical displacement cannot be assessed.
No correlation between gap junctional coupling properties and the density of fibronectin matrix was found for Cx43 knockouts and Cx43 overexpressing CNCs in dye coupling experiments, although an upregulation of gap junction communication with altered integrin-matrix interactions has previously been described for other cell types (Czyz et al. 2005; Lampe et al. 1998; Shanker et al. 2005).
Similar degradation of extracellular matrix by osteoclasts has previously been reported [23].
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