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We then examined the codes and their corresponding passages through an iterative process, grouping them into broad themes and displaying them in analytic matrices, as recommended by Miles [ 23].
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DNA extraction was done using Instagen Matrix (BioRad) as recommended by manufacturer.
The samples were run on the ABI PRISM® 3130xl Genetic Analyzer (Applied Biosystems) using the Applied Biosystems Multi-Capillary DS-30 (Dye Set D) Matrix Std Kit as recommended by the manufacturer.
After washing with 50 mM NaPO4, 65 mM NaCl, 0.01% Tween 80 (pH 6.0), the VLPs were eluted with 50 mM NaPO4, 500 mM NaCl, 0.01% Tween 80 (pH 6.0) and then captured on a Poros EP 250 coupled to bovine fetuin (30 mg fetuin/mL Poros EP 250 matrix)(Desert Biologicals, Australia) as recommended by the manufacturer and equilibrated in 50 mM NaPO4, 150 mM NaCl (pH 6.0).
8 The Matrix – The Wachowski Brothers, 1999 As recommended by: JoannaDark, Patrick Armstrong, Dave Thompson "Does The Matrix not count?" asks Patrick Armstrong.
Assessing transitions between fragmentation classes over time allows to identification of degraded forests by the dynamic process of degradation, supporting a simple matrix approach to forest monitoring as recommended by Bucki et al. [67].
Population structure was estimated from the three principal components of a principal component analysis (PCA) based on a correlation matrix calculated from genetic markers, as recommended by Bradbury et al. (2007).
A phi-X 174 control lane was included in the Solexa run for matrix, phasing, and error rate estimations as recommended by the manufacturer.
Prior to Mantel testing, the geographic distance matrix was log-transformed and the genetic matrix was also transformed [ FST/(1 − FST)] as recommended by Rousset (1997).
Inspection of the correlation matrix revealed the presence of many coefficients of.30 and above also as recommended.
Quantitative polymerase chain reactions were performed using the LightCycler® FastStart DNA Master SYBR Green I kit as recommended by the manufacturer (Roche Applied Science, Meylan, France) with 1 µl of matrix cDNA.
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