After washing twice with PBS, pH 7.4, the remaining matrices were collected and denatured in Laemmli buffer for western blotting.
For these two reasons the connection matrices were collected separately for four epochs of four years each (1998 2001, 2002 2005, 2006 2009 and 2010 2013).
Cells from 3D collagen matrices were collected in lysis buffer (0.9% Triton X-100, 100 μmol/l PMSF and 20 μg/ml aprotinin in PBS).
Sixty collagen matrices were collected in 5 ml homogenization buffer [20 mM HEPES (pH 7.4), 1 mM EDTA, 250 mM sucrose, 20 mM NaCl, 1.5 mM MgCl2, Protease inhibitor cocktail (Roche), phenylmethylsulfonyl fluoride (2 mM), 1 mmol/l Mercaptoethanol, 0.1 mg/ml collagenase] and digested at 4°C.
The free cells and cells leaked from the support matrix were collected by centrifugation at 10,000 rpm for 20 min and dried at 90 °C.
The supernatant, which contained the mitochondrial matrix, was collected.
Supernatant (mitochondrial matrix) was collected and pellet (mitochondrial inner membrane) was resuspended in 100 μl of PP buffer.
The streptavidin-sepharose matrix was collected by short spin and extensively washed with PBS containing 1 mM DTT and 0.5%NP400.5%NP40
As shown in Figure 1(c), the target protein GP73 combined closely with Ni2+-saturated matrix was collected with above 90% purification after the nonspecific parts were eluted with a series of concentrations of imidazole solution in PBS buffer.
The antibody matrix was collected at 700 gav for 1 minute at 4°C, and washed in a fritted column with 20 ml 10 mM phosphate-buffered saline, 1 M NaCl, 80 mM OG, pH 7.4 followed by 20 ml of the same containing 138 mM NaCl.
To study this in more detail, aerosol particles generated by LA-ICP-MS from Al2O3-NPs of all the three sizes from the agarose matrix were collected on an adhesive carbon film placed on a SEM sample carrier approximately half the way between the ablation chamber and the plasma.
