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Note that the covariance matrices and mean values used in this paper are sample covariance matrices and sample means, estimated from the data.
To evaluate contamination from laboratory, sampling matrices, and sample handling, we analyzed field blanks (n = 4 neutrals; n = 3 phenols), batch blanks (n = 5), and matrix blanks (n = 5 phenols, 6 neutrals).
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EAM-1, a proprietary formulation, that enables efficient laser desorption and ionization of glycosylated proteins and proteins in the 15 50 kDa range As suggested by the manufacturer's documents, low regions such as 2000Da to 6000Da are usually ignored due to the large amount of noise and the restrictions caused by the Proteinchip matrices and samples.
The different matrices and sampling points are shown in Figure 1.
Runs were designed to evaluate the role of adsorption buffer composition, feed type (pH, salt content), CPG matrix and sample pretreatment (RNase A, isopropanol precipitation).
Factors including tested strains, concentration, choice of S9 (rodent enzyme) matrix and sample quality all affect the quality of the activity data [25].
Goodness of fit is an indicator for testing the similarity between the model-estimated covariance matrix and sample covariance.
Matrix and sample solution (0.2 μg μl−1) were mixed (1 1 v/v) and applied onto the stainless steel sample plate as 1.5 μl of droplets.
The pronase digests were analyzed on the MALDI TOF mass spectrometer using the same matrix and sample preparation detailed above for the tryptic digest analysis.
Intersample variability caused by using different matrixes and sample loading, however, can lead to shifts in retention times, which complicates the use of retention time for identification purposes.
Selection of projects based on the Justification Matrix and sampling.
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