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We continued to conduct mating trials until we had obtained 40 successful focal matings for each of our treatments.
I have simulated 5000 double matings for each category of s2.
The target number of matings for each group combination is then initiated.
The most time-consuming step is setting up and evaluating 20 single-pair matings for each new insertion line to detect recessive lethality.
Table 2 shows an example calculation of relative weightings (W), used to set the target number of matings for each group combination.
Every two or three generations of selection, embryos from a representative sample of the best matings (for each male, two or more straws of embryos are cryopreserved) are recovered and vitrified.
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Nm (second column for males, second row for females) is the number of matings for which each animal should be used, and this in turn drives selection, including the extent to which each animal is used.
For example, Maxuse = 1 mating for natural mating females, 30 matings for natural mating bulls, 1,000 matings for artificial insemination bulls, or the number of semen doses left for a deceased bull.
In the random mating scheme, all bulls were mated randomly to cows, while limiting the maximum number of matings permitted for each bull (5000).
After PA are adjusted, the bull that produces the highest PA when mated to a cow is selected in a sequential manner, while the number of matings permitted for each bull is constrained to prevent one bull from being mated to all cows.
Typically each male has zero or multiple matings to make, and there is a ranking for each mating, rather than for each male, such that matings for a given male are generally dispersed throughout the ranked list.
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