Indeed, Wegewitz et al. [ 30] showed recently that males are better at mating with strain CB4856 hermaphrodites than with hermaphrodites of the lab-adapted strain N2.
These transformants were mated with strain EGY48 that had been transformed with plasmid pLexA.
Plant tests and plasmid profile investigation by a modified Eckhardt gel procedure confirmed that conjug transfer of the plasmid carrying the symbiosis gene region in strain HAMBI 1207 (streptomycin-resistant derivative of HAMBI 1141) occurred when the nodulation-defective strain HAMBI 1587 (N. galegae sv. orientalis), was mated with strain HAMBI 1207.
The mutation was identified by positional cloning using amplified fragment length polymorphism (AFLP) analysis of the progenies in 48 tetrads produced by mating with S1-D2 strain (Kathir et al., 2003), followed by gene sequencing.
Since, the haploid MATa variant of the hfi1Δ strain was described as hypersensitive to DOX, and both the ctk1Δ and hfi1Δ diploids display enhanced levels of mating with MATα tester strains (see below), we compared the relative sensitivity of the MATa haploid derivatives to DNA damage when compared to the WT counterpart.
And it cross-fertilizes," mating with a different strain of grass and creating a new genetically modified breed.
Prior to screening, the self-activity of each bait clone was tested by mating with the Y187 strain harboring an empty pACT2 vector and then plating on SD/-His/-Leu/-Trp/ medium supplemented with 2.5, 5, or 10 mM 3-amino 1,2,4-triazole (3-AT).
Prior to screening, the self-activity of each bait clone was confirmed by mating with the Y187 strain harboring an empty pACT2 vector and then plating on SD/-His/-Leu/-Trp/ medium supplemented with 2.5, 5, 10, or 50 mM 3-amino 1,2,4-triazole (3-AT).
When they mate with other strains, their winning tickets break up and their offspring are wiped out by the drug.
