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Mating was performed between strains ER-6c HAA1-OP and ER-3a HAA1-OP tobtainin the diploid HAA1-overexpressing strain ER HAA1-OP (MAT a/α).
After emergence of new born individuals brother-sister mating was performed (F1).
Mating was performed by placing females in the cages of males for several days.
To select interacting partners of our proteins of interest, mating was performed using the pools of Mav203 containing pVV213 plasmids and Mav103 strains containing pVV212 bearing our genes of interest.
Triparental mating was performed as previously described [ 80].
Mating was performed by mixing haploids and selecting for diploids on SD TRP LEU plates.
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Mating is performed by dominant males and female members in a social-spider colony.
Yeast protocols (growth and mating) were performed as described in [ 9].
After the heat detection in the bitch, natural mating is performed three times on alternate days.
Broth and filter mating were performed by using E. faecium GE1 as recipient strain (6 ).
Fission yeast growth, gene targeting, and mating were performed as described (Bamps et al., 2004; Fersht et al., 2007).
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