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The occurrence of mating was defined as the male staying in contact with the female for at least 30 sec.
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As re-mating was defined as a binomial variable, the influence of male-male competition, male heterozygosity, and genetic dissimilarity on divorce rate, was analysed using a logistic regression model, controlling for female genotype.
Each mating period was defined as the period between the first mate-guarding day and the last mate-guarding day observed in any of the three groups.
The presence of mating combat was defined as observing aggressive physical contests between individuals of the same sex to gain access to mates or attractive territories [ 26].
A recent study in Arabidopsis showed that female-determined mate choice was defined by four quantitative trait loci (QTLs) (Fitz Gerald et al. 2014), suggesting that the ability to sort among pollen can be genetically determined.
A mate-guarding period was defined as a period of one to several consecutive extensive mate-guarding days.
For each new species designated by molecular sequence analysis and mating experiments, one strain was defined as a reference strain (see below).
Hermaphrodite mating success (r h ) was defined as the relative proportion of hermaphrodites that outcross GFP males at each assay plate (n = 96).
Pregnant mice were obtained following overnight mating (Day of vaginal plug was defined as embryonic day 0.5).
The proportion of outcrossed hermaphrodites within each assay plate was defined as hermaphrodite mating success, while the fertility (number of viable offspring until adulthood) was defined as hermaphrodite fitness under outcrossing or under selfing, depending on the individual breeding status (see data in [ 30] and Methods).
The refractory period was defined as including both reduced mating drive and less-efficient execution of mating.
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