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In Experiment 2, mating was confirmed in 10 out of the 14 cyclic females.
Non-primiparous females from both strains were housed with males overnight and mating was confirmed by the presence of a vaginal plug.
The successful mating was confirmed by the presence of plugs.
Mating was confirmed through a positive sperm smear the following morning.
Successful mating was confirmed by the presence of a spermatophore attached to the female.
In the morning, mating was confirmed by examination of vaginal plugs, and considered 0.5 day post coitum (DPC).
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Heterozygous zebrafish resulting from this mating were confirmed by PCR and mated to spawn cofilin-1 mutant fish.
The former environment minister and internationally acclaimed green activist Marina Silva, who was already Mr Campos's running mate, was confirmed as his successor.
Successfully mated were confirmed by the presence of a vaginal plug before 10 00 h the following morning (day 1 of pregnancy) and were bred individually.
The mating type was confirmed by mating experiments with the C. gattii, serotype C, MATα reference tester strain NIH312 [27] as well as with the MATα supermater strain JF101 [28] (Figure 2).
The same number of mating factors was confirmed present by Kauserud et al. (2006).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com