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Mating was conducted by introducing two females into the cage of a male wildtype conspecific.
Mating was conducted by mixing stationary-phase recipient with late log-phase or stationary-phase donor, pelleting the cells, followed by resuspension in 50 μl of MilliQ water and spotting on a TY plate with 5-aminolevulinic acid (ALA; 50 μg/mL).
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Two types of mating assays, plate and slide mating, were conducted.
Observations for mating were conducted several times each week for a period of 6 8 weeks.
But we unfortunately had chosen the height of the rutting season for our visit, and all night long the plaintive lowing of the stags desperate to find a mate was conducted at a volume that was a barrier to sleep.
Mating analysis was conducted on Murashige and Skoog (MS) media, which contains myo-inositol that stimulates mating [41].
A screen for gene transfer within H. volcanii biofilms through the known mating mechanism was conducted by cultivating biofilms composed of two strains, each with a unique auxotrophic marker.
All hemiclones were kept in individual vials prior to the mating assay, which was conducted on the 13th day of the flies' life (i.e. 3 4 days post-eclosion).
A factorial mating design in two environments was conducted by including nine cytoplasmic male sterile lines (A) and five restorer lines (R) along with their F1 (A×R), F2, reciprocal RF2 and BC1 (A×F1).
A factorial mating design in two environments was conducted using 7 cytoplasmic male sterile lines (A) and 5 restorer lines (R) along with their F1 (A × R) and F2 populations.
Knockout female embryos were obtained by mating heterozygous parents and genotyping was conducted by PCR of tail skin DNAs using mouse Cre and Y chromosome primers.
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