For phenotype assessment, ΔCYC2 strains were starved for 24 h and mixed with equal amount of each mating type of strains to induce meiosis.
The molecular type and mating type of each strain were confirmed by URA5 gene RFLP analysis [12] and mating type specific PCR [8].
Both mating types of P. destructans were found to coexist within hibernacula, suggesting the presence of mating populations in Europe.
STE20 is not only serotype-specific but also mating-type specific (Frasier et al. 2004), which makes it an appropriate marker for the determination of serotype and mating type of C. neoformans.
Chromosome walking was used to determine the full length mating type genes of these species.
The isogenic mating partner, K6001α, was obtained by mating type switching of K6001 induced by a plasmid carrying the wild type HO gene.
In addition to the mating analysis to determine the mating type of each isolate, PCR and sequence analysis were used to detect either of the two mating type-specific idiomorphs of the SXI genes.
Overall, we saw complete mating within group B (all clones of mating type A mated with all of mating type B), and a high degree of mating within the other major clade of groups C, D, E, and F, with a few matings between two major clades.
will help to differentiate between the effect of mating type loci and ecophysiological divergence of two species.
Consequently, mating type dependent regulation of these genes does not appear to be abolished.
The mating types of the isolates were determined by multiplex PCR amplification by using 2 pairs of mating type locus (MTL specific primers.
