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The programmed pause sites at the mating type switch locus and the ribosomal DNA repeats promote recombination.
The yeast orthologue of Tim, Swi1, is required for stabilizing replication forks at some DNA polymerase pause sites, like those found at the mating type switch locus (Rts1) and centromeres, where replication terminates [11], [12], [57].
And in yeast, will GamGFP be able to detect the breaks that are induced to initiate a mating type switch?
This possibility is suggested by quantitative analyses of MAPK regulation of the mating type switch in yeast.
Khd1p is involved in the asymmetric localization of Ash1p in daughter cells, which is a transcription inhibitor of the mating type switch protein encoded by the HO gene.
However, a simple mating type switch proved to be insufficient to enable sexual reproduction within the T. reesei QM6a strain line [ 12].
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It was first identified in yeast as a gene involved in mating type switching [20], but is now known to be a highly conserved gene in organisms ranging from archea to humans [21].
Using genomic data from two populations of S. paradoxus, Tsai et al. (2008) estimated that when mating occurs, 94% of the time it is intratetrad mating, 5% of the time it is mating type switching, and the remaining 1% of the time it is outcrossing.
Correct regulation of heterochromatin silencing is essential for efficient mating type switching.
Inheritance of mating type switching is asymmetric due to a strand specific and site-specific imprint.
Furthermore, Mec1 is known to activate the SCF/UFO1 complex to degrade HO, an endonuclease involved in mating type switching.
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