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For phenotype assessment, ΔCYC2 strains were starved for 24 h and mixed with equal amount of each mating type of strains to induce meiosis.
The molecular type and mating type of each strain were confirmed by URA5 gene RFLP analysis [12] and mating type specific PCR [8].
STE20 is not only serotype-specific but also mating-type specific (Frasier et al. 2004), which makes it an appropriate marker for the determination of serotype and mating type of C. neoformans.
A multiplex PCR test for determining mating type of the pathogens Tapesia yallundae and Tapesia acuformis is described.
The test was used successfully to determine the mating type of 118 isolates of T. yallundae and T. acuformis from Europe, North America, and New Zealand.
MAT-specific PCR was used to rapidly determine the mating type of isolates of A. rabiei sampled from chickpea fields in the US Pacific Northwest.
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Both mating types of P. destructans were found to coexist within hibernacula, suggesting the presence of mating populations in Europe.
Chromosome walking was used to determine the full length mating type genes of these species.
The isogenic mating partner, K6001α, was obtained by mating type switching of K6001 induced by a plasmid carrying the wild type HO gene.
Overall, we saw complete mating within group B (all clones of mating type A mated with all of mating type B), and a high degree of mating within the other major clade of groups C, D, E, and F, with a few matings between two major clades.
will help to differentiate between the effect of mating type loci and ecophysiological divergence of two species.
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