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In nature, it is expected that a few white cells would spontaneously switch and function as a source of pheromone of the opposite mating type, namely the product of MFα (Yi et al. 2011a).
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A final question arises about the selective factor(s) that could have led to the origin and maintenance of mating type polymorphism, namely selfing/outcrossing in Bulbophyllum clade C. In this context, it is worth recalling the two main but mutually non-exclusive models about the short-term selective advantages of selfing in flowering plants (Charlesworth, 2006; Cheptou, 2012; Wright et al., 2013).
This coincides with the timing of replication of centromeres, which in S. pombe differs from the timing of replication of the other major constitutive heterochromatin domains, namely telomeres and mating type loci (Chen et al., 2008; Kloc et al., 2008).
Of the single spore isolates from strain V23, 101were confirmed as homokaryons, 35 of which were mating type A1 and 66 mating type A2.
Of the single spore isolates from strain PY, 72 were confirmed as homokaryons, of which 41 were mating type A3 and 31 mating type A4.
Furthermore, the potential of mating type gene sequences for species delimitation was evaluated.
Chromosome walking was used to determine the full length mating type genes of these species.
The remaining 23 were heterokaryons carrying both A1 and A2 mating type loci.
Sixteen were heterokaryons carrying both A3 and A4 mating type loci.
In both budding yeast and fission yeast, sex change occurs when copies of epigenetically silent mating type information replace expressed mating type information.
‡ –, mating type unknown.
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