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Furthermore, the potential of mating type gene sequences for species delimitation was evaluated.
Amplification and sequence analysis of the sex specific SXI1α mating type gene, as well as the absence of the mating type specific SXI2a gene, confirmed that both isolates had the α mating type allele (MATα).
This would test the importance of mating type gene expression in a different way.
The inability of amicronucleates to mate may be the result of error(s) in mating type gene rearrangement.
Because mating type gene order is largely conserved among basidiomycetes, positional homology can be used in the search for the mating type genes.
Among these are 274 conserved hypothetical proteins, hypothetical, or predicted proteins, but also several ribosomal protein genes, histone genes, a mating type gene, and the frequency gene that is central to the cell's circadian rhythm.
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Chromosome walking was used to determine the full length mating type genes of these species.
Phylogenies based on mating type genes could discriminate between the species analysed and are in concordance with the results obtained with the more conventional multilocus phylogenetic analysis approach.
Since this pathway is usually activated in response to the presence of a compatible mating partner, the need for mating type genes involved in pheromone recognition and homeodomain protein compatibility could be bypassed, and eventually the pheromone/pheromone receptor genes might even be lost from the genome.
Furthermore, we also put forward the existence of an alternative model in which even in the absence of mating type genes epistatic or genetic changes altering the regulation of the downstream components of the cascades controlling mating and filamentation and could result in the observed life cycle of F. depauperata (supporting File S1).
The fission yeast Schizosaccharomyces pombe contains heterochromatin at centromeres, subtelomeres, and mating type genes, as well as at small islands of meiotic genes dispersed across the genome.
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