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The Leupold strain 968 (h mating type) corresponds to CBS1042, whereas Leupold strains 972 (h -S mating type) and 975 (h +N mating type) are spontaneously occurring heterothallic derivatives of CBS1042 (Munz et al. 1989).
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Of the single spore isolates from strain V23, 101were confirmed as homokaryons, 35 of which were mating type A1 and 66 mating type A2.
Of the single spore isolates from strain PY, 72 were confirmed as homokaryons, of which 41 were mating type A3 and 31 mating type A4.
In both budding yeast and fission yeast, sex change occurs when copies of epigenetically silent mating type information replace expressed mating type information.
The molecular type and mating type of each strain were confirmed by URA5 gene RFLP analysis [12] and mating type specific PCR [8].
‡ –, mating type unknown.
It is of the h + mating type.
*MLST, multilocus sequence typing; MAT, mating type; CSF, cerebrospinal fluid.
In mating confrontations using H. jecorina mutants and the female sterile T. reesei QM6a both hpr1 and hpr2 were shown to be mating type specifically required for mating.
Both octads showed the Mendelian segregation pattern for mating type: four mated with Sj2, and the other four mated with Sj3.
The transformants were allowed to switch mating type, mate and homozygous diploids were picked and tested for sporulation and mating [ 37].
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