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Most chromosomes, including the mating type chromosome, have the potential to be lost.
*M-Sla1H: haploid hybrid genotype with a1 mating type chromosome originating from M. lychnidis-dioicae; M-Sda2H: haploid hybrid genotype with a2 mating type chromosome originating from M. silenes-dioicae (and vice versa for M-Sda1H and M-Sla2H).
The binding-site of the primer is within the sequence belonging to the STE-20 gene on the mating type chromosome [ 61].
For clarity of presentation, haploid meiotic products obtained from the F1-hybrids are named according to their mating type, the origin of their mating type chromosome, and superscript "H" to indicate their hybrid origin.
The serotype/mating type of the remaining seven AD hybrid isolates could not be determined because of the loss of the mating type chromosome of one serotype (Table 1).
For hybrid isolates where the mating type chromosome originated from M. silenes-dioicae (M-SdH), the more frequent infection was the backcross to the mating-type heterospecific M-Sl rather than to the homospecific M-Sd, but the differences were not statistically significant (2-tailed Binomial-test, M-Sda1H: N = 52, p = 0.68; M-Sda2H: N = 67, p = 0.46) (Table 2).
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The results show significant support for pre-mating barriers depended on the combinations of specific mating type chromosomes and less obvious signs for post-mating isolation driven by the origins of the mating types.
In Microbotryum, the MAT region is located on a pair of non-recombining and size-dimorphic mating type chromosomes [ 25].
The use of a novel host environment did, however, allow our study to assess mating compatibility based upon the species-specific mating type chromosomes.
In brief, 11 12% of random genomic markers was heterozygous and linked to mating type, while the mating-type chromosomes themselves represent only ∼13% of the total genome size.
The dispersal of mating type loci to different chromosomes has also been found in A. gossypii, whereas in E. cymbalariae all three mating type loci are located on chromosome 1 (Wendland and Walther 2005, 2011; Dietrich et al. 2013).
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