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Transcriptional activation of mating-related genes, formation of mating projections, and fusion of two opposite mating type cells are involved in this process.
Transcriptional activation of mating-related genes, formation of mating projections, and fusion of the two opposite mating type cells are involved in this process.
Same-sex mating, also called monokaryotic fruiting, occurs in same mating type cells, predominantly in the C. neoformans MATα strains [30].
A third library contains clones derived from "sexually competent cells" (cells are cultured in liquid medium in the dark, and are competent for fusion with opposite mating type cells), cells that are roughly equivalent to growth phase cells.
The timing for collection of samples for RNA was chosen based on earlier observations on the timing of gamete formation: under the specified experimental set up, pairing cells could be observed starting from 10 h after the opposite mating type cells were mixed together and gametes could be observed 24 h after the opposite mating type cells were mixed together (Scalco et al. in press).
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Fertilization occurs when a specialized hyphae (trichogyne) from the protoperithecium fuses with a cell from an opposite mating type cell (male).
How dynamics at different levels can be described in a rule-based approach has been shown with a model of fission yeast to analyze the regulations between cell cycle control, cell division, mating type switching, and cell-cell communication via diffusible pheromone molecules.
Each of mating type a cells (WT [ URA3 ], sir2 ∆ [ URA3 ], and sir2 ∆ hml ∆ [ URA3 ]) and α cells (BY4742) was seeded together into YPD medium at an initial OD600 = 0.2 and then incubated at 30°C for 1 day.
Because both CBS7001 and CBS1502 contain a wild-type HO, any a/ a or α/α spores will switch mating type following cell division and then self-fertilize to produce tetraploids, while the a/α spores would remain as stable, nonmating diploids.
Interestingly, Yi et al. (2011b) discovered recently that C. albicans biofilms formed by MTL (for mating type locus -heterozygous celocus -heterozygousicellsy in permeability andifferedesignificantly thein MTL-homozygous counterpermeability
However, although the P-factor-specific protease Sxa2 lowers the amount of P-factor pheromone and thus lowers the effects on M cells, mating type switching ensures equal distributions of cells with mating types P and M. The model so far assumes all cells as well as each secreted pheromone molecule residing in the same solution, i.e. there is no distinction between different locations.
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