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Among these 37 genes were 7 innate immune response genes, consistent with the known ability of mating to induce the innate immune response.
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Spellman et al. [ 2] used temperature-sensitive mutants of cdc15 and cdc28, as well as stimulation by the α-factor mating pheromone, to induce synchrony in budding yeast (S. cerevisiae).
In addition, we used CO2-narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation.
Eight-week-old females were mated to BALB/c males to induce pregnancy and inspected every morning for vaginal plugs.
In closely related mealworm beetles (Tenebrio molitor) mating was found to induce the down-regulation of immune function [ 41].
For phenotype assessment, ΔCYC2 strains were starved for 24 h and mixed with equal amount of each mating type of strains to induce meiosis.
Incidental observations confirmed this was sufficient to induce mating.
Either a blue stroboscopic light source (Opaluz strobe warming light [30 W]) 22 or an automated 40 lumens white light flashlight (Opalux Flash 40 LED) 21 was used to shine into the mosquito cages to induce mating.
Although the age of the genus is not well established, early work by Kniep (1926) demonstrated some limits of the Microbotryum pheromone to induce mating structures in other fungi.
All remaining clones were homozygous at all three markers, but 372 clones were homozygous for the Isolate B alleles, while only three were homozygous for the Rosetteless alleles, raising the possibility that the Rosetteless mutant was less viable than Isolate B under the starvation conditions used to induce mating.
Prior to mating, induce ovulation of female frogs with 20 IU human chorionic gonadotropin (hCG) at night before in vitro fertilizations.
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