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All mating tests were videotaped in real-time for subsequent behavioral scoring.
The order of aggression and mating tests were counter-balanced across animals.
If the morphology of a new isolate was not unique, mating tests were performed with individuals of morphologically similar strains.
After that, additional three mating tests were conducted with sexually receptive females to determine the baseline sexual activity of the rats.
Mating tests were conducted as described above (see Sexual Behavior) 4h after progesterone treatment.
Mating tests were conducted at three different nutrient levels; 10%, 50%, or 100% of the level recommended by the manufacturer to test the affect of nutrients on zygospore germination.
Similar(53)
This mating test was artificially divided into three groups according to the male's ages as follows: 35 60 days, 61 100 days, and older than 100 days.
Mate choice tests were conducted when males were about six months old.
Mate choice tests were conducted under natural light conditions on a large terrace closed with mesh wire and a plexiglas roof, in an aviary (185×185×185 cm) equipped with food, water and natural branches (Fig. 4b).
Although tabulated as "choices," the primary data we recorded from our mate-choice tests were each the outcome of a contest [ 61].
Given the prediction that speciation will yield positive-assortative mating, one-tailed tests were appropriate.
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