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Experiment 3 was identical to Experiment 2 except that female subjects received no baseline mating test and were tested for mate choice 24 h and 6 days after their last injection.
Student's t-test was performed for body weight, testis weight, sperm count, mating test, chromosomal asynapsis, and the number of MLH1 foci.
In order to assess the effect of mate choice during these mating encounters on reproductive success, one male rat in each pair was categorized as the preferred mate if the female spent more time (> 50%) with him during the mating test of the present study.
In each mating test inocula from two isolates were placed 1 cm from each other on a fresh Petri plate.
The ploidy status of the F2 segregants was checked in a mating test [ 26].
Each mating test used 50 OX3097D-Bol males, 50 wild males, and 50 wild females.
Similar(48)
However, with a growing number of species, mating tests become tedious and time-consuming.
MAF collected a large number of strains used in this study and performed mating tests.
MA, MVR and JBP contributed to mating tests and MA to acquisition of sequence data.
In this study, species were initially identified via mating tests (Additional File 1).
Complementary mating-type locus sequencing, mating tests, and culturing in the presence of elm terpenes were conducted.
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