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"I'm thrilled that Academy visitors will have the opportunity to view this fascinating animal up close in the aquarium, where they'll see just why its beauty, unique mating technique and social habits are intriguing the cephalopod community," said Richard Ross, a biologist at the California Academy of Sciences, in a statement.
The plasmid was delivered via conjugation with S17-1λpir by using a membrane filter mating technique.
The filter mating technique was carried out as previously described [ 18].
14 Both studies underscored the importance of cage size and density of specimens per experiment to obtain fertilized females and avoid the forced mating technique.
The resulting strains were assessed for conjugative transfer frequency using the drop-plate mating technique described previously (Farrand et al. 2002; Wetzel et al. 2014).
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No matter how uncomfortable the passionate smooches (or whatever they are) made passersby, at least it's not a weird as some of these mating techniques.
Standard mating techniques [36], [38] were then used to produce, initially, mouse strains that contained a "tri-lox" Ate1 configuration, in that they also contained the floxed positive-selection PPGK-hph cassette (Fig. 2D).
In order to evaluate whether the blaOXA-48 gene could be transferred, conjugation experiments were performed using a standardized method for liquid mating techniques, as described elsewhere [ 13].
23 In our case, forced mating techniques with this species were unsuccessful, with visual forced mating confirmed but spermathecae found to be negative for insemination when visualized under dark-field microscope (data not shown).
Screening of the Schistosoma library was performed by using the yeast-mating technique.
The forced-mating experiments were carried out using the artificial-mating technique described in Ow-Yang et al [ 17].
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