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First, a diribonucleotide at the S. pombe mating switch locus is believed to be the signal initiating homologous recombination (Vengrova and Dalgaard, 2004).
Replication fork stalling may occur in regions that contain clustered ribonucleotides, as seen at the S. pombe mating switch locus (Vengrova and Dalgaard, 2006).
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The programmed pause sites at the mating type switch locus and the ribosomal DNA repeats promote recombination.
The yeast orthologue of Tim, Swi1, is required for stabilizing replication forks at some DNA polymerase pause sites, like those found at the mating type switch locus (Rts1) and centromeres, where replication terminates [11], [12], [57].
Replication fork arrest is also used to control the direction of replication through a site in the mating locus that carries an imprint that controls which daughter cell will undergo a mating switch event (Dalgaard and Klar 2001).
The molecular markers that we used had two haplotypes (alleles) at each of the two gene loci, mitochondrial intermediate peptidase (MIP), which is closely linked to mating type locus A in other studied mushrooms (James et al. 2004), and pheromone receptor (**RCB), which is a region within mating type locus B in other mushrooms (Aimi et al. 2005; Brown and Casselton 2001).
Sixteen were heterokaryons carrying both A3 and A4 mating type loci.
The remaining 23 were heterokaryons carrying both A1 and A2 mating type loci.
Additionally, transcription factors MATα1 and MATα2, the yeast mating type loci, contain an SCD and bind SCD-containing Mcm1, further suggesting additional targets for Tel1/Mec1 during mating type switching.
Correct regulation of heterochromatin silencing is essential for efficient mating type switching.
Inheritance of mating type switching is asymmetric due to a strand specific and site-specific imprint.
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