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To represent genetic variance in female responsiveness, female responsiveness was measured as the mean mating speed of each female hemiclone line across mean male hemiclone lines.
In a similar study, the male x female genotype interaction contributed to 38.1% of the variance observed in mating speed, suggesting that the mating speed of males was strongly influenced by the genetic identity of the female they courted [ 31].
The mean mating speed of each male hemiclone line (based on mating speed obtained with each of the other 11 female hemiclone lines) was used to calculate male attractiveness (with longer times to mate indicating "less attractive" males ([ 45]).
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By mating males to two different female genotypes (low receptivity vs. high receptivity), it appeared that the expression of mating speed in both absolute and relative performance of male genotypes in D. melanogaster was strongly influenced by the female genotype [ 47].
To estimate the additive genetic variation seen among all 12 of our hemiclone lines we partitioned the variance of mating speed, copulation duration, and egg size for block effect, male genetic identity, female genetic identity, and the interaction of the two.
The significant MxF for mating speed from our estimates of variance components using the complete analysis is likely due to our data set, and not experimental design.
Using an REML approach we were able to quantify the extent to which phenotypic variation in mating speed was dependent on genetic identity of one or both sexes.
Genetic incompatibilities as a result of outbreeding may lead to variance in mating speed and other pre-copulatory traits.
Female choosiness was calculated as the coefficient of variance (CV) and was obtained by calculating the standard deviation of the mean mating speed for female hemiclone lines (calculated by obtaining the mean mating speed value for each female hemiclone line mated with each male hemiclone line and averaged across experimental block) [ 7].
Differences in the specific genetic identity of males and females both individually (but not jointly) had a significant effect on the variation in mating speed.
The reduction in harmful matings evolved because of changes in the speed of mating, with faster maters mating with other compatible fast maters, slow maters mating with other compatible slow maters, thus reducing harmful mating.
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