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In the hermaphrodite L. stagnalis we find high costs of mating for both mating roles.
For the focal individuals mating roles were noted and egg masses produced during both day (immediately upon laying) and night (next morning) were collected for egg counting.
The number of males and females available for mating, and thus mating roles and the proportion of singing males, can vary over relatively short geographical distances due to microhabitat differences between populations of this flightless species [ 27].
When both mating partners are motivated to donate, mating roles can be swapped after the first insemination [ 50]. Adult animals from a three-months-old cohort were collected from our mass rearing culture and housed individually in perforated plastic jars in a large tank with running low-copper water under standard lighting conditions (light:dark = 12 12).
As male densities dwindled, the fish completely reversed their conventional mating roles, the researchers report in the current issue of The American Naturalist.
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After removing the non-significant interaction term (treatment × mating role) from the model, we found that mating role (donor or recipient) had a significant effect on prostate dry weight, while treatment did not (ANCOVA on residual prostate dry weight: F1,70 = 4.50, P = 0.04 and F2,70 = 0.33, P = 0.72, for mating role and treatment, respectively).
In both the defensive (i.e. first to mate) and offensive (i.e. second male to mate) roles, males from the polygamous lines gained exclusive paternity of 33% of the litters, while males from the monogamous lines gained exclusive paternity of just 14% of the litters.
For instance, mating experience could affect their mating role decision (Hermann et al. 2009).
Thus, animals that were restricted to one mating role did not perform that role more often than reciprocating hermaphrodites.
Nonetheless, if a particular mating role is favoured (e.g. first rather than last), a male is still predicted to invest more in the favoured role [ 14].
Therefore, combinations of H and F result in pairs that can only copulate in one mating role: H as donor and F as recipient.
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