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For subsequent library screening, the mating protocol was followed (Clontech).
The deficiency strains were put through the same mating protocol as the point mutations to reduce or eliminate background effects.
Mating protocol consisted in housing two females with one male in transparent high temperature polysufone cages (26.7×20.7×14.0 cm) with water and food available ad libitum.
The mating protocol has been described elsewhere (Fromont-Racine et al, 1997).
The mating protocol was performed and mating rates were calculated as described recently [ 18].
Each combination of strain and mating protocol was tested in triplicate.
Similar(52)
Conversely, numerous instances have been documented whereby insects show kin preference, based on a whole range of processes which include preferred mating protocols, acoustic and visuals clues and pheromone detection.
Mate pair libraries were generated using the Nextera Mate Pair protocol (Illumina, San Diego, CA).
The last 3 8 kb libraries were prepared following Nextera mate pair protocol, following manufacturer's instructions.
In each year class, 109 341 full-sib families were produced via nested paternal or partial factorial mating protocols.
To investigate whether the hypothalamus of the intact adult zebrafish contains potential GnRH progenitors we used adult male zebrafish that had been selected via the mating training protocol and sacrificed them to obtain brains for sectioning.
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