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Evolutionary biologists had built this kind of model before to understand mating preferences in other animals, but we added some new ingredients.
Clearly we still have much to learn about the neural mechanisms of species recognition and mating preferences in túngara frogs.
In addition, mating preferences in zebra finches are substantially influenced by early learning about visual characteristics [48], including morphological novelties [49] [51].
Since all matings took place in dark climate chambers, it seems unlikely that colour would interfere with mating preferences in this study.
These types of behavioral studies inspired Wilczynski et al. [17] to propose that mating preferences in the túngara frog result from a simple summation of the acoustic energy in the call.
Spermatophore production is limited in the few species where male reproductive rates have been studied [35], [33], and sex ratios are typically female biased [36] making mating preferences in males feasible.
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However, association time is an approved measure for mating preference in sticklebacks [ 26- 28].
Most experimental-evolution studies of reproductive isolation have focused on premating isolation and mating preference in Drosophila [ 3, 15, 16].
Furthermore, other recent studies found that mating preference in cichlids may be based partly on olfactory cues (Plenderleith et al. 2005; Verzijden and ten Cate 2007).
Studies utilizing D. serrata have, for example, investigated the evolution of sexual dimorphism in CHCs [ 12, 13], divergence of mating preference in novel environments [ 14] and post-copulatory sexual selection [ 15].
Accordingly, it is easy to conceive that mating preferences diverge in two isolated populations.
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