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Evaluation of mating ability was performed by cultivating yeast cells with a mating partner in 1 ml YPD medium at 30 °C for 1.5 h.
We therefore analysed how this strain would behave in the presence of a mating partner in terms of pheromone precursor and pheromone receptor gene regulation.
We therefore cultivated QM6a under equal conditions as for the mating experiments of the wild-type CBS999.97 described above and investigated the transcriptional response to the presence of a mating partner in this cross.
With a mating partner in one of the neighbouring sites, a surviving vegetative cell may enter the sexual cycle with probability s turning itself and a randomly chosen, suitable neighbour into gametes, and mate.
Under disassortative mating, individuals with rare cues might tend to have a mating partner in better condition than themselves and vice versa, potentially improving the relative fitness of individuals with rare recognition alleles.
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Both estimates of polyandry (number of alleles, and maximum likelihood number of mating partners), were consistent with a smaller number of mating partners in androchrome females [ 36] Figure 2, although the estimate for maximum likelihood number of mating partners per morph type should be interpreted with caution since it is based on a small sample size of 3 individuals.
These three models also included as a covariate the total number of matings scored on each day of the rut to control for between-day variation in the availability of mating partners in our population.
Moreover, they adjust the timing of dispersal from the nest both to the availability of mating partners in the nest and the presence of ergatoid fighter males [ 33].
We also determined the Bateman gradient (the relative number of mating partners in relation to the relative number of clutches) for successfully reproducing males and females (cf. [ 39]).
Finding the best combination of mating partners in mating programs that are based on genomic information requires time- and memory-intensive computing because of the large amount of data.
Generally, in sexually reproducing Ascomycetes mating partners of two opposite mating-types (in S. cerevisiae a or α) are attracted by a pheromone signal relay that leads to cell fusion, karyogamy, and formation of diploid nuclei that can undergo meiosis and spore formation.
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