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We found that the mean litter size, 3.3±1.4, of Coil −/− mating pairs was significantly smaller than that of wild-type mating pairs, 6.9±2.2 (Figure 1A).
Accounting for these pseudo mating pairs was necessary to ensure Mendelian segregation at fog-2 under selfing.
The effect on mating pairs was only present in the two exposure treatments with parasites, and not in the bacterial treatment or control (figure 1 inset; Dunnett's test: natural p < 0.001, parasite p = 0.013).
Importantly, the average litter size of the wild-type C57BL6/J mating pairs was in good agreement with published data for C57BL6/J mice [36].
(3) Minimized covariance of ancestral contributions mating (MCAC), in which contributions of each ancestor to each candidate were calculated and the covariance between the contributions for the proposed set of male and female mating pairs was minimised [ 2].
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Non-mating flies were removed and mating pairs were left until the next day.
During the rainy season, males become territorial, and mating pairs are established.
In H. argenteocinereus, paternity analyses demonstrated that burrow systems of mating pairs were several hundred meters away from each other in some cases.
In all figures, the number of egg laying groups or mating pairs is shown in brackets.
Subpopulations were maintained separately for 50 generations, when mating pairs were selected and total of ~1700 RILs were created [ 103].
As haplodeficiency of hmmr did not induce fertility defects, hmmr +/m mating pairs were used as controls.
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