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dit mutants were obtained by conventional mating of carriers or gynogenesis (Goda et al., 2006).
Since no new rams from other farms have joined the flock in recent years, the most likely reason for this high frequency for a lethal allele will be inbreeding leading to an increased proportion of the flock being carriers of the putative autosomal recessive trait and hence increase the likelihood of mating of carriers leading to birth of affected lambs.
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For decades, genetic carrier testing has referred primarily to testing with the aim of identifying heterozygote carriers of mutations that put a mating of two carriers at a one in four risk of having a child with a disorder causing serious disability or early death.
P aa) will be equal to either 0.25, for a mating of two carriers, 0.5, for a mating of an affected animal with a carrier, or 1, for a mating of two affected animals.
The line is maintained by brother × sister mating of heterozygous carriers, and is now at generation N10.
The insemination success in risk matings was compared with the insemination success in non-risk matings (non-carrier bulls mated to daughters of carrier sires) using a linear regression model.
However, due to the avoidance of carrier mating, we unfortunately lacked tissue from more mutant calves for further analysis.
Very little is known about this family of carriers, although a previous meta-analysis of microarray data identified some MATE transporters among a list of stress-induced transporters [ 39].
Identification of carriers is crucial as much as breeder awareness about the avoidance of certain mates in order to prevent economic loss for the horse industry.
Besides the experimental mating of an affected male to known disease carriers [20], [21], the parents of all available cases were healthy (Figure S1).
Polled animals that had been produced from a mating of horned and polled parents were obligate carriers, as were animals that produced horned progeny from matings to horned animals.
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