Sentence examples for mating mixtures were from inspiring English sources

After 40 minutes of incubation, mating mixtures were plated onto agar containing streptomycin (100 mg/l) and ceftazidime (1 mg/l).

Following matings the controls and mating mixtures were scraped off of the TYG agar plates and re-suspended in 3 ml of sterile 1×PBS, serially diluted and plated in duplicate on TYG agar supplemented with tetracycline and erythromycin for selection of the transconjugants.

The timing and level of filamentation in the middle parts of diluted mating mixtures were recorded and compared.

Second, after cells were mated on SPA the mating mixtures were resuspended and washed with 1% glucose, rather than H2O.

To determine the number of donors and recipients the mating mixtures were also plated onto TYG containing either erythromycin (donors) or tetracycline (recipients).

Mating mixtures were digested with glusulase overnight to eliminate nonspore cells.

After cultivation overnight at 30°C, the mating mixture was plated onto YPD agar.

The mating mixture was subsequently resuspended in 500  μl of sterile PBS before plating onto selective agar (TYG+erythromycin).

The mating mixture was digested with glusulase and spores were purified using a Percoll gradient as described (Sun et al. 2013).

Third, after treatment of mating mixtures with glusulase, the spore suspensions were treated with a lower final concentration of ethanol (15%) than previously employed (30%).

The mixtures were then spotted in 50 μl spots onto 2%% water agar and allowed to mate for 2 days at 14 °C.